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Okay the problem wasn't the router.

I spoke to the ISP earlier today and they insisted they do not/are not blocking port 25.

I called up again today and I am now being told that they ARE blocked at the ISP level.

One of the machines was infected and causing issues, and that machine has been removed from the network and is being checked over, however, had the ISP told me in the first place that they were blocking I would not have looked for issue elsewhere.

I thank everybody here for the assistance and appologise if I wasted time, especially to sc302, again thanks a lot for all your help.

That usually doesn't happen....unless you are on a dynamic ip address.

Still though, you would be on a black list database somewhere and everything looked clean.

I know, which is why I believed them when they said we weren't being blocked.

The ISP level blocking must have come in to place before any other spam traps were activated.

I'll be suggesting they leave this ISP soon :p

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